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<h1 id="firstHeading" class="firstHeading mw-first-heading"><span class="mw-page-title-main">RIP-Chip</span></h1>
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<div id="mw-content-text" class="mw-body-content mw-content-ltr" lang="de" dir="ltr"><div class="mw-content-ltr mw-parser-output" lang="de" dir="ltr"><p>Der <b>RIP-Chip</b> (engl. <i><span lang="en">RNA immunoprecipitation Chip</span></i>, ‚RNA-Immunpräzipitations-Microarray‘) ist eine Methode der <a href="Biochemie" title="Biochemie">Biochemie</a> zum Nachweis von <a href="RNA-Protein-Interaktion" class="mw-redirect" title="RNA-Protein-Interaktion">RNA-Protein-Interaktionen</a>. Der RIP-Chip ist eine Kombination aus einer <a href="Immunpr%C3%A4zipitation" title="Immunpräzipitation">Immunpräzipitation</a> eines RNA-bindenden Proteins mit einer <a href="RT-PCR" class="mw-redirect" title="RT-PCR">RT-PCR</a> und einem <a href="Microarray" title="Microarray">Microarray</a> und dient zur Untersuchung der verschiedenen gebundenen RNA eines solchen Proteins.<sup id="cite_ref-1" class="reference"><a href="#cite_note-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup>
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<div class="mw-heading mw-heading2"><h2 id="Prinzip">Prinzip</h2></div>
<p>Durch eine Immunpräzipitation eines bekannten, meist <a href="Rekombinantes_Protein" title="Rekombinantes Protein">rekombinanten</a> Proteins, können aus einer RNA-Lösung spezifisch <a href="Affinit%C3%A4t_(Biochemie)" title="Affinität (Biochemie)">bindende</a> RNA-Sequenzen angereichert und aufgereinigt werden. Diese RNA werden anschließend von dem Protein getrennt und durch RT-PCR in cDNA umgeschrieben. Diese erzeugte cDNA wird zur <a href="Hybridisierung_(Molekularbiologie)" title="Hybridisierung (Molekularbiologie)">Hybridisierung</a> mit DNA auf einem Microarray eingesetzt.<sup id="cite_ref-2" class="reference"><a href="#cite_note-2"><span class="cite-bracket">[</span>2<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-3" class="reference"><a href="#cite_note-3"><span class="cite-bracket">[</span>3<span class="cite-bracket">]</span></a></sup>
</p><p>Der RIP-Chip besitzt Ähnlichkeiten zum <a href="ChIP-on-Chip" title="ChIP-on-Chip">ChIP-on-Chip</a>, welcher mit ähnlichem Prinzip gebundene <a href="DNA-Sequenz" class="mw-redirect" title="DNA-Sequenz">DNA-Sequenzen</a> DNA-bindender Proteine charakterisiert. Die <a href="CLIP-Seq" title="CLIP-Seq">CLIP-Seq</a>, <a href="PAR-CLIP" title="PAR-CLIP">PAR-CLIP</a> und <a href="ICLIP" title="ICLIP">iCLIP</a> sind Alternativverfahren zum RIP-Chip mit <a href="DNA-Sequenzierung" title="DNA-Sequenzierung">DNA-Sequenzierungen</a> im <a href="Hochdurchsatz" class="mw-redirect" title="Hochdurchsatz">Hochdurchsatz</a> anstelle des Microarrays.
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<div class="mw-heading mw-heading2"><h2 id="Einzelnachweise">Einzelnachweise</h2></div>
<ol class="references">
<li id="cite_note-1"><span class="mw-cite-backlink"><a href="#cite_ref-1">↑</a></span> <span class="reference-text">Townley-Tilson WH, Pendergrass SA, Marzluff WF, Whitfield ML: <cite style="font-style:italic">Genome-wide analysis of mRNAs bound to the histone stem–loop binding protein</cite>. In: <cite style="font-style:italic">RNA</cite>. 12. Jahrgang, <span style="white-space:nowrap">Nr.<span style="display:inline-block;width:.2em"> </span>10</span>, Oktober 2006, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>1853–67</span>, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1261/rna.76006">10.1261/rna.76006</a></span>, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/16931877?dopt=Abstract">PMID 16931877</a>, <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1581977/">PMC 1581977</a> (freier Volltext).<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rfr_id=info:sid/de.wikipedia.org:RIP-Chip&rft.atitle=Genome-wide+analysis+of+mRNAs+bound+to+the+histone+stem-loop+binding+protein&rft.au=Townley-Tilson+WH%2C+Pendergrass+SA%2C+Marzluff+WF%2C+...&rft.date=2006-10&rft.doi=10.1261%2Frna.76006&rft.genre=journal&rft.issue=10&rft.jtitle=RNA&rft.pages=1853-67&rft.pmc=1581977&rft.pmid=16931877&rft.volume=12.+Jahrgang" style="display:none"> </span></span>
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<li id="cite_note-2"><span class="mw-cite-backlink"><a href="#cite_ref-2">↑</a></span> <span class="reference-text">Khalil AM, Guttman M, Huarte M, <i>et al.</i>: <cite style="font-style:italic">Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression</cite>. In: <cite style="font-style:italic">Proc. Natl. Acad. Sci. U.S.A.</cite> 106. Jahrgang, <span style="white-space:nowrap">Nr.<span style="display:inline-block;width:.2em"> </span>28</span>, Juli 2009, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>11667–72</span>, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1073/pnas.0904715106">10.1073/pnas.0904715106</a></span>, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/19571010?dopt=Abstract">PMID 19571010</a>, <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2704857/">PMC 2704857</a> (freier Volltext).<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rfr_id=info:sid/de.wikipedia.org:RIP-Chip&rft.atitle=Many+human+large+intergenic+noncoding+RNAs+associate+with+chromatin-modifying+complexes+and+affect+gene+expression&rft.au=Khalil+AM%2C+Guttman+M%2C+Huarte+M%2C+...&rft.date=2009-07&rft.doi=10.1073%2Fpnas.0904715106&rft.genre=journal&rft.issue=28&rft.jtitle=Proc.+Natl.+Acad.+Sci.+U.S.A.&rft.pages=11667-72&rft.pmc=2704857&rft.pmid=19571010&rft.volume=106.+Jahrgang" style="display:none"> </span></span>
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<li id="cite_note-3"><span class="mw-cite-backlink"><a href="#cite_ref-3">↑</a></span> <span class="reference-text">D. G. Hendrickson, D. J. Hogan, D. Herschlag, J. E. Ferrell, P. O. Brown: <i>Systematic identification of mRNAs recruited to argonaute 2 by specific microRNAs and corresponding changes in transcript abundance.</i> In: <i>PloS one.</i> Band 3, Nummer 5, 2008, <span class="-print"><a href="Internationale_Standardnummer_f%C3%BCr_fortlaufende_Sammelwerke" title="Internationale Standardnummer für fortlaufende Sammelwerke">ISSN</a> <span style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://zdb-katalog.de/list.xhtml?t=iss%3D%221932-6203%22&key=cql">1932-6203</a></span></span>, S. e2126, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.pone.0002126">10.1371/journal.pone.0002126</a></span>. <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/18461144?dopt=Abstract">PMID 18461144</a>, <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2330160/">PMC 2330160</a> (freier Volltext).</span>
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